Real-time quantitative PCR
The full program can be downloaded here
| 8:35 |  |
welcome address |
| Jo Vandesompele (Ghent University, Belgium) | ||
| Session 1 | TECHNICAL ADVANCES - NEW APPLICATIONS (I) | |
| chair : Hakim El Housni (Erasme University Hospital, Brussels, Belgium) | ||
| 8:45 | |
High-Resolution DNA Melting Analysis |
| Carl Wittwer (University of Utah, USA) | ||
| 9:10 | Immuno-qPCR : Novel Opportunities in Clinical Diagnostics and Research | |
| Christof Niemeyer (University of Dortmund, Germany) | ||
| 9:35 | Direct detection of rare circulating tumor cells in blood by competitive allele-specific TaqMan-based PCR (castPCR) | |
| Caifu Chen (Applied Biosystems, Foster City, USA) | ||
| 9:50 | |
Quantitative one-step DNA methylation analysis using native genomic DNA as template |
| Thomas von Kanel (Department of Pediatrics, University of Bern, Switzerland) | ||
| 10:05 | coffee break | |
| Session 2 | STANDARDIZATION - OPTIMIZATION & VALIDATION | |
| chair : Jan Hellemans (Ghent University, Belgium) | ||
| 10:35 | |
MIQE : a 9 month update |
| Stephen Bustin (University of London, UK) | ||
| 11:00 | |
From reference genes to global mean normalization |
| Jo Vandesompele (Ghent University, Belgium) | ||
| 11:25 | |
Normalization of gene expression in cell lines by reference and other stably expressed genes using RealTime ready qPCR assays |
| Rudolf Mihalik (University of Semmelweis, Romania) | ||
| 11:50 | |
Development of RQS : a novel metric for the assessment of RNA integrity |
| Elaine Wong-Ho (Caliper Life Sciences, USA) | ||
| 12:10 |
| Measurable impact of RNA quality on gene expression results from quantitative PCR |
| Katleen De Preter (Ghent University, Belgium) | ||
| 12:25 | lunch + poster session | |
| Session 3 | EXPRESSION PROFILING - BIOMARKERS | |
| chair : Stephen Bustin (University of London, UK) | ||
| 14:00 |
| Single cell qPCR expression correlation |
| Mikael Kubista (University of Prague, Czech republic) | ||
| 14:25 |
| An integrative genomics screen uncovers ncRNA T-UCR functions in neuroblastoma |
| Pieter Mestdagh (Ghent University, Belgium) | ||
| 14:40 | |
Challenges of using qPCR for eQTL mapping |
| Ellen De Keyser (Institute for Agricultural and Fisheries Research, Melle, Belgium) | ||
| 14:55 | |
The Fluidigm dynamic gene expression array revealed embryo-maternal tolerance mechanisms in the bovine endometrium prior to implantation |
| Suzanne Ulbrich (Technical University of Munich, Germany) | ||
| 15:10 |
| RNA pre-amplification enables large-scale RT-qPCR gene-expression studies on limiting sample amounts |
| Stefaan Derveaux (Ghent University, Belgium) | ||
| 15:25 | coffee break | |
| Session 4 | TECHNICAL ADVANCES - NEW APPLICATIONS (II) | |
| chair : Jo Vandesompele (Ghent University, Belgium) | ||
| 16:05 | |
A qPCR based method (using TaqMan Protein Expression Assays) for detection of proteins in human germ cell tumors |
| Leendert Looiienga (University of Rotterdam, the Netherlands) | ||
| 16:30 |
| Fast qPCR assay optimization and validation techniques for HTS |
| Francisco Bizouarn (Bio-Rad, Hercules, USA) | ||
| 16:55 |
| Recent advances and new perspectives in RT-qPCR |
| Michael Pfaffl (University of Munich, Germany) | ||
| 17:20 | awards | |
| Hakim El Housni (Erasme, University Hospital, Brussels, Belgium) | ||
| 17:30 | drinks | |